https://en.wikipedia.org/wiki/TUNEL_assay
TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. [3] The assay relies on the use of terminal deoxynucleotidyl transferase (TdT), an enzyme that catalyzes attachment of deoxynucleotides, tagged with a fluorochrome or another

https://www.abcam.com/kits/tunel-staining
Learn how to use TUNEL staining (or TUNEL assay) to detect DNA breaks in apoptosis. Compare different TUNEL staining methods, their advantages and disadvantages, and the most popular TUNEL staining kits.

https://www.news-medical.net/whitepaper/20190225/TUNEL-Staining-an-Overview.aspx
Principles of TUNEL Staining. Terminal deoxynucleotide transferase (TdT) is an enzyme that is expressed in certain immune cells. As it attaches deoxynucleotides to the 3'-hydroxyl terminus of

https://pubmed.ncbi.nlm.nih.gov/22566045/
Abstract. Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template.

https://rwu.pressbooks.pub/encyclopediaofbiologicalmethods/chapter/tunel-assay/
Learn how to use TUNEL assay to label cells with extensive DNA fragmentation, often used to mark apoptotic cells. The assay involves fixing, solubilizing, and staining samples with TdT and labeled dUTP.

https://www.jove.com/v/5651/the-tunel-assay
Learn how to use the TUNEL assay to detect apoptotic cells by labeling fragmented DNA with fluorescent dUTPs. This video covers the principles, protocol, and applications of this technique in tissue sections.

https://www.sciencedirect.com/topics/neuroscience/tunel-assay
TUNEL fluorescence assay is a well-established, fast, and simple nonradioactive technique to detect and quantify neurons undergoing apoptosis. It detects free 3′-OH termini in single-stranded breaks in high-molecular-weight nuclear DNA fragments. The presence of these groups is considered an established marker of apoptosis.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808282/
Combines immunohistochemistry and TUNEL assay to measure cell death. •. Marks the fragmented DNA to quantify dying cells in specific regions of the tissue. •. This protocol can be adapted to assay cell death in other tissues as well. Cell death maintains tissue homeostasis by eliminating dispensable cells. Misregulation of cell death is

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795088/
Abstract. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell

https://pubmed.ncbi.nlm.nih.gov/33401733/
Abstract. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell death

https://www.news-medical.net/life-sciences/What-is-TUNEL-Staining.aspx
Terminal deoxynucleotidyl transferase dUTP nick end labeling, also known as TUNEL staining or the TUNEL assay, is a robust technique for the detection of apoptosis. This kind of cell death is

https://journals.sagepub.com/doi/full/10.1177/002215549804600306
TUNEL was performed with the Boehringer Mannheim Kit. Bar = 5 μm. Figure 3 Scheme of TUNEL patterns and pitfalls in relation to tissue treatment. Apoptosis is characterized by strong DNA compaction and a high degree of DNA fragmentation. Tissue fixation, i.e., protein and DNA crosslinking and denaturation, hinders TUNEL reagent access to the 3

https://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/apoptosis-protocol/tunel-protocols/click-it-tunel-alexa-fluor-imaging-assay-protocol.html
Learn how to use the Click-iT TUNEL Alexa Fluor imaging assay to detect apoptosis in adherent cells grown on coverslips or a 96-well plate. The assay utilizes a bio-orthogonal click reaction between an alkyne-modified nucleotide and an azide-modified fluorophore.

https://www.promega.com/products/cell-health-assays/apoptosis-assays/deadend-fluorometric-tunel-system/
A classic TUNEL Assay to detect and quantitate apoptotic cells in cultured cells or FFPE tissue sections. The assay measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP using Terminal Deoxynucleotidyl Transferase (TdT).

https://link.springer.com/protocol/10.1385/1-59259-213-9:141
Proceed with the TUNEL Assay for Adherent Cells (Subheading 3.5.2.). Process all positive control slides in separate coplin jars to avoid introducing DNaseI contamination into experimental samples. 3.5 TUNEL Assay 3.5.1 Cell Suspensions for Flow Cytometry. 1. Centrifuge 1 × 10 6 cells, remove supernatant, and resuspend cells in 1 mL PBS

https://link.springer.com/protocol/10.1007/978-1-61779-860-3_5
Learn how to use terminal deoxynucleotidyl transferase (TdT) to label apoptotic cells with DNA breaks in situ. This protocol describes a colorimetric TUNEL assay for mouse embryonic dental tissues with positive and negative controls.

https://www.sciencedirect.com/topics/medicine-and-dentistry/tunel-assay
TUNEL assay is a method to detect apoptosis in tissue sections by labeling DNA fragments produced by endonucleases. Learn about the limitations, applications, and protocols of TUNEL assay from various chapters and articles on ScienceDirect Topics.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2295206/
Compared with other TUNEL variants the Br-dU-labeling assay offers the greatest sensitivity in detecting DNA breaks. Described also are modifications of the protocol that allow one to use other than Br-dUTP fluorochrome-tagged deoxynucleotides to label DNA breaks. Concurrent staining of DNA with propidium or 4′,6-diamidino-2-phenylindole

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/imaging-apoptosis-assays/tunel-assays-dna-fragmentation-chromatin-condensation.html
Learn how to use TUNEL assays and DNA stains to detect apoptosis in cells and tissues. Compare different methods, such as click chemistry, fluorescence, and colorimetric, and see examples of protocols and results.

https://pubmed.ncbi.nlm.nih.gov/33260475/
The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3'-hydroxyl termini. Given that genomic DNA breaks arise during early and late stages of apoptosis, TUNEL staining continues to be widely used as a measure of apoptotic cell death. The advantages of the assay include

https://www.rndsystems.com/resources/technical/tunel-assay-principle
TUNEL Assay Principle. DNA fragmentation represents a characteristic hallmark of apoptosis. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is an established method for detecting DNA fragments. The TACS® TdT kits contain a highly purified form of the TdT enzyme for the enzymatic incorporation of biotinylated nucleotides.

https://www.eltiempo.com/cultura/gente/como-prevenir-el-efecto-tunel-al-conducir-consejos-y-recomendaciones-3355679
Los ojos poseen una excelente visión periférica, pero al conducir a altas velocidades se pierde la capacidad de ver los lados.

https://adevarul.ro/stiri-externe/europa/cel-mai-lung-tunel-rutier-si-feroviar-subacvatic-2370808.html
Danemarca și Germania sunt cu un pas mai aproape de a fi legate prin cel mai lung tunel rutier și feroviar subacvatic din lume. Acesta va reduce durata călătoriei de la 45 de minute, cât durează în prezent traversarea cu feribotul, la doar șapte minute cu trenul.

https://caracol.com.co/2024/06/25/por-mantenimiento-habra-cierres-nocturnos-del-tunel-de-oriente-del-24-al-28-de-junio/
La Concesión Túnel Aburrá Oriente informó que para realizar mantenimiento del Túnel de Oriente se realizarán cierres totales de 10 p.m. a 4 a.m. del 24 a la madrugada del 28 de junio.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730366/
Abstract. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3ʹ-hydroxyl termini. Given that genomic DNA breaks arise during early and late stages of apoptosis, TUNEL staining continues to be widely used as a measure of apoptotic cell death.

https://www.20minutos.es/motor/movilidad/tunel-ferroviario-catesby-circulan-coches-toda-velocidad-5433326/
Situado a nueve metros de profundidad en el Valle del automovilismo, en este paso subterráneo antes circulaban ferrocarriles, pero ahora lo hacen superdeportivos de todo tipo. Además, cualquiera

https://g1.globo.com/rj/rio-de-janeiro/noticia/2024/06/24/carro-pega-fogo-e-interdita-tunel-reboucas-sentido-centro.ghtml
Um carro pegou fogo no início da noite desta segunda (24), na primeira galeria do Túnel Rebouças, sentido Centro. A via ficou interditada por cerca de 1h15, até ser liberada, por volta das 20h15.

https://www.20minutos.es/motor/movilidad/antiguo-tunel-ferroviario-catesby-circulan-coches-toda-velocidad-5433326/
Así es el imponente túnel de Catesby. El túnel de Catesby se construyó a finales del siglo XIX, en plena época victoriana, y formó parte de la red ferroviaria británica durante casi siete

https://www.mdpi.com/1422-0067/22/1/412
Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell death in cultured kidney cells and animal and human kidneys

https://caracol.com.co/2024/06/24/el-tunel-de-oriente-tendra-cierres-nocturnos-esta-semana/
Desde hoy lunes, 24 de junio, y hasta el próximo jueves, 27 de junio, se tendrán cierres nocturnos en el Túnel de Oriente, que une al Valle de Aburrá, con el Valle de San Nicolás y el