https://en.wikipedia.org/wiki/TUNEL_assay
TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. [3] The assay relies on the use of terminal deoxynucleotidyl transferase (TdT), an enzyme that catalyzes attachment of deoxynucleotides, tagged with a fluorochrome or another

https://www.abcam.com/kits/tunel-staining
Learn how to use TUNEL staining (or TUNEL assay) to detect DNA breaks in apoptosis. Compare different TUNEL staining methods, their advantages and disadvantages, and the most popular TUNEL staining kits.

https://pubmed.ncbi.nlm.nih.gov/22566045/
Abstract. Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template.

https://www.news-medical.net/whitepaper/20190225/TUNEL-Staining-an-Overview.aspx
Principles of TUNEL Staining. Terminal deoxynucleotide transferase (TdT) is an enzyme that is expressed in certain immune cells. As it attaches deoxynucleotides to the 3'-hydroxyl terminus of

https://rwu.pressbooks.pub/encyclopediaofbiologicalmethods/chapter/tunel-assay/
Learn how to use TUNEL assay to label cells with extensive DNA fragmentation, often used to mark apoptotic cells. The assay involves fixing, solubilizing, and staining samples with TdT and labeled dUTP.

https://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/apoptosis-protocol/tunel-protocols/click-it-tunel-alexa-fluor-imaging-assay-protocol.html
Learn how to use the Click-iT TUNEL Alexa Fluor imaging assay to detect apoptosis in adherent cells grown on coverslips or a 96-well plate. The assay utilizes a bio-orthogonal click reaction between an alkyne-modified nucleotide and an azide-modified fluorophore.

https://journals.sagepub.com/doi/full/10.1177/002215549804600306
TUNEL was performed with the Boehringer Mannheim Kit. Bar = 5 μm. Figure 3 Scheme of TUNEL patterns and pitfalls in relation to tissue treatment. Apoptosis is characterized by strong DNA compaction and a high degree of DNA fragmentation. Tissue fixation, i.e., protein and DNA crosslinking and denaturation, hinders TUNEL reagent access to the 3

https://link.springer.com/protocol/10.1007/978-1-61779-860-3_5
Learn how to use terminal deoxynucleotidyl transferase (TdT) to label apoptotic cells with DNA breaks in situ. This protocol describes a colorimetric TUNEL assay for mouse embryonic dental tissues with positive and negative controls.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2295206/
Compared with other TUNEL variants the Br-dU-labeling assay offers the greatest sensitivity in detecting DNA breaks. Described also are modifications of the protocol that allow one to use other than Br-dUTP fluorochrome-tagged deoxynucleotides to label DNA breaks. Concurrent staining of DNA with propidium or 4′,6-diamidino-2-phenylindole

https://link.springer.com/article/10.1007/s00418-024-02306-9
TUNEL is the most sensitive, accurate, and quantitative test (Moore et al. 2021), applicable to different biological samples. In this study, we aim to conduct a comparative analysis of DNA fragmentation in different cell lines using the TUNEL assay on metaphase chromosomes samples, nuclei and cellular structures recovered from cultures media.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795088/
Abstract. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell

https://www.news-medical.net/life-sciences/What-is-TUNEL-Staining.aspx
Terminal deoxynucleotidyl transferase dUTP nick end labeling, also known as TUNEL staining or the TUNEL assay, is a robust technique for the detection of apoptosis. This kind of cell death is

https://link.springer.com/protocol/10.1385/1-59259-213-9:141
Proceed with the TUNEL Assay for Adherent Cells (Subheading 3.5.2.). Process all positive control slides in separate coplin jars to avoid introducing DNaseI contamination into experimental samples. 3.5 TUNEL Assay 3.5.1 Cell Suspensions for Flow Cytometry. 1. Centrifuge 1 × 10 6 cells, remove supernatant, and resuspend cells in 1 mL PBS

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/imaging-apoptosis-assays/tunel-assays-dna-fragmentation-chromatin-condensation.html
Learn how to use TUNEL assays and DNA stains to detect apoptosis in cells and tissues. Compare different methods, such as click chemistry, fluorescence, and colorimetric, and see examples of protocols and results.

https://www.sciencedirect.com/topics/neuroscience/tunel-assay
TUNEL fluorescence assay is a well-established, fast, and simple nonradioactive technique to detect and quantify neurons undergoing apoptosis. It detects free 3′-OH termini in single-stranded breaks in high-molecular-weight nuclear DNA fragments. The presence of these groups is considered an established marker of apoptosis.

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/tunel-assay
Learn about the TUNEL assay, a technique to detect DNA fragmentation in cells that undergo apoptosis or necrosis. Find out how TUNEL is used in various fields of biochemistry, genetics, and molecular biology, such as neural stem cells, tooth tissue cryopreservation, and islet transplantation.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808282/
Combines immunohistochemistry and TUNEL assay to measure cell death. •. Marks the fragmented DNA to quantify dying cells in specific regions of the tissue. •. This protocol can be adapted to assay cell death in other tissues as well. Cell death maintains tissue homeostasis by eliminating dispensable cells. Misregulation of cell death is

https://www.rndsystems.com/resources/technical/tunel-assay-principle
TUNEL Assay Principle. DNA fragmentation represents a characteristic hallmark of apoptosis. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is an established method for detecting DNA fragments. The TACS® TdT kits contain a highly purified form of the TdT enzyme for the enzymatic incorporation of biotinylated nucleotides.

https://www.sciencedirect.com/topics/medicine-and-dentistry/tunel-assay
TUNEL assay is a method to detect apoptosis in tissue sections by labeling DNA fragments produced by endonucleases. Learn about the limitations, applications, and protocols of TUNEL assay from various chapters and articles on ScienceDirect Topics.

https://pubmed.ncbi.nlm.nih.gov/33260475/
The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3'-hydroxyl termini. Given that genomic DNA breaks arise during early and late stages of apoptosis, TUNEL staining continues to be widely used as a measure of apoptotic cell death. The advantages of the assay include

https://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/apoptosis-protocol/tunel-protocols.html
Protocols. Click-iT® TUNEL Alexa Fluor® Imaging Assay Protocol. LIVE/DEAD® Fixable Dead Cell Stain KitsLIVE/DEAD® Violet Viability/Vitality Kit.

https://cnnespanol.cnn.com/2024/06/25/tunel-vial-ferroviario-mas-largo-mundo-mar-baltico-trax/
(CNN) -- Con un descenso de hasta 40 metros bajo el mar Báltico, el túnel sumergido más largo del mundo unirá Dinamarca y Alemania, reduciendo drásticamente la duración de los trayectos

https://pubmed.ncbi.nlm.nih.gov/33401733/
Abstract. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell death

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730366/
Abstract. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3ʹ-hydroxyl termini. Given that genomic DNA breaks arise during early and late stages of apoptosis, TUNEL staining continues to be widely used as a measure of apoptotic cell death.

https://www.elnacional.cat/es/sociedad/cerrado-tunel-bracons-problemas-estructura-viaducto_1243329_102.html
Cerrado el túnel de Bracons, que une las comarcas de la Garrotxa y Osona por un esfrondament, según ha informado el Servei Català de Trànsit (SCT).La C-37 también se encuentra tajada por problemas en la estructura del viaducto entre la Vall d'en Bas y Torelló.

https://www.mdpi.com/1422-0067/22/1/412
Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3'-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell death in cultured kidney cells and animal and human kidneys

https://www.cmjornal.pt/portugal/detalhe/incendio-em-viatura-dentro-do-tunel-do-marao-corta-circulacao-no-sentido-amarante---vila-real
Um incêndio numa viatura de mercadorias, dentro do Túnel do Marão, obrigou ao corte da circulação no sentido Amarante - Vila Real. A informação foi confirmada ao Correio da Manhã por fonte do Comando Sub-regional de Emergência e Proteção Civil do Tâmega e Sousa. De acordo com a mesma

https://apnews.com/sports/deportes-eurocopa-2024-83917138f2f9731f3cc38f2c8482b436
DÜSSELDORF, Alemania (AP) — Cristiano Ronaldo evitó por muy poco ser golpeado por un aficionado que salto desde las gradas para acercarse al astro durante el encuentro entre Portugal y Georgia el miércoles en el Campeonato Europeo.

https://g1.globo.com/rj/rio-de-janeiro/noticia/2024/06/28/homem-e-flagrado-usando-chinelo-amarrado-a-corda-para-furtar-cabos-do-tunel-noel-rosa.ghtml
Um homem foi flagrado arrebentando a fiação do Túnel Noel Rosa, em Vila Isabel, na Zona Norte do Rio. Na quinta-feira (20), a câmera de segurança capturou o homem usando um chinelo amarrado a

https://www.voaportugues.com/a/tendas-de-esperan%C3%A7a-s%C3%A3o-luz-ao-fundo-do-t%C3%BAnel-para-toxicodependentes-no-lubango/7676440.html
Em Angola existem pelo menos 22.500 consumidores de drogas, com o álcool e cannabis sativa (vulgo liamba) no topo, de acordo com dados de 2022 do Instituto Nacional de Luta Anti-droga, que alerta também para o crescimento no consumo do craque e da cocaína.