https://www.youtube.com/channel/UCJI5E_owUtziCekJmpRbdDA
Just a guy making electronic vocaloid music for fictional characters 😎🇵🇸 A mishmash of electronic, video game, nerdcore, & synthetic vocals. New song eve

https://xtramath.org/
A Solid Math Foundation. XtraMath is an online math fact fluency program that helps students develop quick recall and automaticity of basic math facts. Students with a strong foundation have greater confidence and success learning more advanced math like fractions and algebra. Create a Free Account. Go Premium.

https://pubmed.ncbi.nlm.nih.gov/30530516/
Here, in vivo cloning of E. coli by XthA is referred to as in vivoE. coli cloning (iVEC). We also show that iVEC activity is reduced by deletion of the C-terminal domain of DNA polymerase I (PolA). Collectively, these results suggest the following mechanism of iVEC. First, XthA resects the 3' ends of linear DNA fragments that are introduced

https://www.nature.com/articles/s41598-022-07878-x
XthA is known as a multi-functional enzyme, and its homologues are involved in the DNA repair system in various bacterial species. Nonetheless,

https://www.neb.com/en/products/m0206-exonuclease-iii-e-coli
Exonuclease III (. E. coli. ) Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA. Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379578/
As a result, in the ΔxthA mutant, a sufficient number of transformants comparable to the number in the xthA + strain were obtained from the DNA fragments with overhangs, whereas DNA fragments with blunt ends yielded few recombinants (Fig. 2E). Hybridization between homologous single-stranded DNA regions of the introduced DNA fragments

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238392/
Mutants in xthA are hypersensitive to hydrogen peroxide and near -UV light . Exonuclease III is controlled by the stationary phase and general stress response sigma factor, RpoS , and is required for the resistance to peroxide that is induced as cells begin to enter to stationary phase. Exonuclease III is also required for resistance to chain

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046245/
The xthA recA and xthA lexA double mutants are extraordinarily sensitive to AZT, at doses as low as 5 ng/ml (Figure 6 A and B, note lower dose scale), levels much greater than the single mutants. The xthA ruvC mutant was one of the most sensitive strains examined in this study, with over 5-orders of magnitude of killing at the low dose of 15 ng

https://genius.com/Xtha-chromatica-lyrics
Guarantee your heart is a plaything. Waiting to tear apart. Draw a line and wait for the ending. Unintended, mended and sewn. Everyday's a little longer when you're all alone. [Chorus] And the

https://www.semanticscholar.org/paper/Exonuclease-III-(XthA)-Enforces-In-Vivo-DNA-Cloning-Nozaki-Niki/29c7a3480fdbedf6fedd8f61437519beee9bcd15
First, XthA resects the 3′ ends of linear DNA fragments that are introduced into E. coli cells, resulting in exposure of the single-stranded 5′ overhangs. Then, the complementary single-stranded DNA ends hybridize each other, and gaps are filled by DNA polymerase I. Elucidation of the iVEC mechanism at the molecular level would further

https://www.uniprot.org/uniprotkb/P09030/entry
xthA. Status. UniProtKB reviewed (Swiss-Prot) Organism. Escherichia coli (strain K12) Amino acids. 268 (go to sequence) Protein existence. Evidence at protein level. Annotation score. 5/5. Entry. Variant viewer. Feature viewer. Genomic coordinates. Publications. External links. History.

https://www.researchgate.net/publication/5812464_XthA_Exonuclease_III_regulates_loading_of_RecA_onto_DNA_substrates_in_log_phase_Escherichia_coli_cells
XthA is known as a multifunctional DNA repair enzyme that has important biological roles in DNA metabolism (phosphatase, exonuclease, AP endonuclease, RNase H activities) 8,9 .

https://www.youtube.com/watch?v=VliKbv7aeqg
If you couldn't tell by the title… this might have been made as a counter to my other song Chromatica… I wanted to do some edm for Error with a mix of confus

https://www.biorxiv.org/content/10.1101/454074v1
First, XthA resects the 3′ ends of linear DNA fragments that are introduced into E. coli cells, resulting in exposure of the single-stranded 5′ overhangs. Then, the complementary single-stranded DNA ends hybridize each other, and gaps are filled by DNA polymerase I. Elucidation of the iVEC mechanism at the molecular level would further

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901738/
Purified XthA from Stellar cell extract is sufficient for general DNA assembly. Motohashi previously reported that DNA assembly can be performed using E. coli cell extract 18, a method named SLiCE assembly 19.While a number of enzymes could be responsible for SLiCE assembly and in vivo recombination activity in E. coli 20, recent reports revealed the important role of XthA and its homologues

https://pubmed.ncbi.nlm.nih.gov/18034795/
Mutations in xthA (ExoIII), xseAB (ExoVII), xni (ExoIX), exoX (ExoX) and tatD (ExoXI) increased the number of RecA-GFP foci twofold to threefold in a population of log phase cells grown in minimal medium. These increases depend on xonA. Epistasis analysis shows that ExoVII, ExoX, ExoIX and ExoXI function in a common pathway, distinct from

https://www.researchgate.net/publication/329560112_Exonuclease_III_XthA_Enforces_In_Vivo_DNA_Cloning_of_Escherichia_coli_To_Create_Cohesive_Ends/fulltext/5c87f584299bf14e7e781c7c/Exonuclease-III-XthA-Enforces-In-Vivo-DNA-Cloning-of-Escherichia-coli-To-Create-Cohesive-Ends.pdf
This indicates that plasmid maintenance and DNA uptake are normal in the ΔxthA strain. Since XthA (exonuclease III) has 3= to 5= exonuclease activity (16), we speculated that resection of the DNA

https://febs.onlinelibrary.wiley.com/doi/full/10.1046/j.1432-1033.2003.03548.x
Additionally the xthA gene was cloned with BspLU11l/PstI in NcoI/PstI cut pETMini vector resulting in pETMini_Af_Exo. pETMini is a version of pETBlue™-2, which lacks a 1155-bp region between the PvuII and SspI sites of the original plasmid resulting in a size of pETMini of 2498 bp. Expression of the xthA gene product of A. fulgidus

https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2007.06026.x
XthA mutants were shown to have the largest increase in the number of RecA‐GFP foci and that this increase was RecB(CD)‐dependent. Consistent with this, xthA recBCD mutants have twofold more DSBs than recBCD mutants alone. The increase in RecA‐GFP foci in xthA mutants was shown to be independent of the BER proteins Nth, MutM and Nei

https://www.researchgate.net/figure/Purified-XthA-is-sufficient-for-DNA-assembly-a-Purified-XthA-proteins-were-verified-by_fig1_359069613
Terms and conditions apply. Purified XthA is sufficient for DNA assembly. (a) Purified XthA proteins were verified by 10.0% SDS PAGE. Lane 1 (left), protein marker; Lane 2, Purified XthA. (b

https://www.coldwellbanker.com/co/hartsel/4662-xtha-trail/lid-P00800000GaxMFL8Yh1GUvXzqLfgfF52aToUF89H
4662 Xtha Trl. Discover your perfect off-grid retreat on this 5.40-acre parcel in Hartsel, Colorado. Featuring level to gently rolling hills, this property offers a serene setting with full sun exposure and stunning surrounding mountain views. Bordering BLM land, it provides a unique opportunity for endless outdoor adventures right at your

https://www.ncbi.nlm.nih.gov/gene/946254
XthA resects the 3' ends of linear DNA fragments that are introduced into Escherichia coli cells, resulting in exposure of the single-stranded 5' overhangs. This increased MMS sensitivity was not suppressed by the presence of multi-copy holC. Addition of the yoaA disruption increased the methyl methanesulfonate (MMS) sensitivity of xthA single

https://journals.asm.org/doi/10.1128/jb.00660-18
As a result, in the ΔxthA mutant, a sufficient number of transformants comparable to the number in the xthA + strain were obtained from the DNA fragments with overhangs, whereas DNA fragments with blunt ends yielded few recombinants . Hybridization between homologous single-stranded DNA regions of the introduced DNA fragments regardless of 5

https://www.instagram.com/suzeet_xtha_/reel/C8bwaYgSph9/
suzeet_xtha_ on June 20, 2024: " ️‍啕 #instagramreels #reels #fypp . . . . . . . . . #fyp #instagram #fyp #fypシ #foryou #foryoupage #goviral #viral #reels #

https://www.ncbi.nlm.nih.gov/Structure/cdd/COG0708
Cn3D. Drawing: All Atoms Virtual Bond. Aligned Rows: up to 5 up to 10 up to 20 up to 40 All 65 rows. Download Cn3D. COG0708 is classified as a model that may span more than one domain. COG0708 is the only member of the superfamily cl43245. Sequence Alignment.