https://www.youtube.com/watch?v=CFUgaxRSq8Q
RNA is a critical component to identify and test for the presence of SARS-CoV-2, the virus that causes COVID-19. RNA is isolated from samples and then PCR i

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640895/
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent of the COVID-19 pandemic. Although other diagnostic methods have been introduced, detection of viral genes on oro- and nasopharyngeal swabs by reverse-transcription real time-PCR (rRT-PCR) assays is still the gold standard. Efficient viral RNA extraction is a

https://www.nature.com/articles/s41467-020-18611-5
The currently widely used procedure for COVID-19 testing involves: a Collection of patient material and deposition of potential SARS-CoV-2 viral particles in transport medium.b Inactivation of the

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780671/
Disease dynamics and optional diagnostic tests for COVID-19. Because of virus load and antibody dynamics, it is necessary to adopt different detection strategies at different time points. Before symptom onset, the optimal choice is nucleic acid testing because its limit of detection (LOD) can reach 10 2 -10 3 copies/ml. After symptom onset

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898297/
Laboratory-based SARS-CoV-2 molecular assays are currently the reference standard for the diagnosis of this infection [ 6 ]. However, point-of-care diagnostic tests and technologies are emerging. In this review, we provide an overview of the molecular diagnostic assays used to diagnose COVID-19, describe their importance in the control of this

https://wi.mit.edu/news/why-rna-research-has-been-critical-during-covid19-pandemic
The molecule comprises the genetic information of SARS-Cov-2, the virus that causes the COVID19 disease, as well as two of the first vaccines being used to provide immunity against the virus. Since early in 2020, researchers at Whitehead Institute and around the world have been applying their RNA knowledge to the COVID19 pandemic.

https://www.sciencedirect.com/science/article/pii/S0003269723003755
This surge in testing capacity worldwide led to shortages in the supply of essential items, including RNA extraction kits, which are crucial for detecting COVID-19 infections. To address this scarcity, researchers have proposed alternative and cost-effective strategies for RNA extraction, utilizing both chemical and physical solutions and

https://www.nature.com/articles/s41467-020-18611-5.pdf
methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples. Our data, including benchmarking using 597 clinical

https://pubmed.ncbi.nlm.nih.gov/33534838/
Mitigating the risk of reopening will require widespread SARS-CoV-2 testing, which would be greatly facilitated by simple, rapid, and inexpensive testing methods. This study evaluates several protocols for RNA extraction and RT-qPCR that are simpler and less expensive than prevailing methods. First, isopropanol precipitation is shown to provide

https://pubmed.ncbi.nlm.nih.gov/33428641/
To respond to the urgent need for COVID-19 testing, countries perform nucleic acid amplification tests (NAAT) for the detection of SARS-CoV-2 in centralized laboratories. Real-time RT-PCR (Reverse transcription-Polymerase Chain Reaction), used to amplify and detect the viral RNA., is considered, as the current gold standard for diagnostics.

https://www.nih.gov/news-events/news-releases/nih-scientists-develop-faster-covid-19-test
Scientists at the National Institutes of Health (NIH) have developed a new sample preparation method to detect SARS-Cov-2, the virus that causes COVID-19. The method bypasses extraction of the virus' genetic RNA material, simplifying sample purification and potentially reducing test time and cost. The method is the result of a collaboration

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246647
Re-opening of communities in the midst of the ongoing COVID-19 pandemic has ignited new waves of infections in many places around the world. Mitigating the risk of reopening will require widespread SARS-CoV-2 testing, which would be greatly facilitated by simple, rapid, and inexpensive testing methods. This study evaluates several protocols for RNA extraction and RT-qPCR that are simpler and

https://newsnetwork.mayoclinic.org/discussion/science-saturday-the-science-behind-the-test-for-the-covid-19-virus/
In the case of COVID-19, researchers have published more than 100 genomes collected from patients to identify key features of the virus that causes that disease, SARS-CoV-2. But there's a wrinkle. PCR only works on DNA, and the COVID-19 virus uses RNA as its genetic code. RNA is similar to DNA, but only has a single strand.

https://www.nature.com/articles/s41598-021-81487-y
The COVID-19 pandemic has resulted in an urgent need for a rapid, point of care diagnostic testing that could be rapidly scaled on a worldwide level. We developed and tested a highly sensitive and

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161125/
Here, we propose a simple, reliable pooling strategy for COVID-19 testing using clinical nasopharyngeal (NP) and/or oropharyngeal (OP) swabs. The strategy includes the pooling of 10 NP/OP swabs for extraction and subsequent testing via quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), and may also be applied to

https://science.mit.edu/3-questions-how-covid-19-tests-work-and-why-theyre-in-short-supply/
A: There are three main types of tests. The first uses the detection of nucleic acid. These tests directly test for the RNA genome of the virus in a variety of sample types, such as nasopharyngeal swabs or sputum. These tests are most commonly performed using polymerase chain reaction (PCR), which can amplify a small part of the virus RNA

https://www.pall.com/de/de/laboratory/blog/covid-19-viral-rna-extraction.html
RNA Extraction for SARS-CoV-2 Detection . As soon as the virus responsible for COVID-19 was identified, scientists began working on ways to detect it. RNA extraction is key to this effort. RNA extraction technology is a critical component of COVID-19 research, vaccine development, patient testing, and community wastewater surveillance.

https://covid19testingtoolkit.centerforhealthsecurity.org/basics/types-of-covid-19-tests/diagnostic-tests/molecular-tests
Molecular Tests. Molecular tests amplify bits of viral RNA so that viral infection can be detected using a specialized test. These tests also are referred to as nucleic acid amplification tests ( NAAT ). The procedure begins by taking a sample from a potentially infected person's nose or mouth (saliva), where virus might be found.

https://www.science.org/content/product/rna-extraction-covid-19-diagnostic-testing
A large number of current COVID-19 diagnostic tests are reliant on detection of viral RNA from buccal swabs by PCR. The column has been adapted to routinely improve yield and quality of viral RNA from nasal and throat swab samples. It provides a fast procedure for downstream RNA extraction, achieving great results in just minutes.

https://www.nature.com/articles/s41598-020-73616-w
Metrics. The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step

https://www.mcgill.ca/oss/article/medical-critical-thinking/blood-microbiome-probably-not-real
Messenger RNA (or mRNA) acts as a disposable copy of a gene, a template for the production of a specific protein. Transfer RNA (or tRNA) actually brings the building blocks of a protein to where they are being assembled. And ribosomal RNA (or rRNA) is the main component of the giant protein factories in our cells known as ribosomes.

https://www.pnas.org/doi/10.1073/pnas.2403063121
We recently showed that the T1TAs timPR is conserved in enterobacteria ().The timP mRNA, initially annotated as noncoding RNA RyfA (), encodes a small toxic inner membrane protein that, upon overexpression, induces membrane leakage and cell growth inhibition ().A puzzling peculiarity of the timPR system was that timP mRNA did not undergo ribonucleolytic processing (), suggesting that

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231976/
Therefore, we have studied the performance and cost-effectiveness of assays/kits employed in SARS-CoV-2 RNA gene detections in samples from suspected cases of COVID-19 in Sao Paulo, Brazil, and observed that one of the bottlenecks in such diagnosis is the shortage of RNA extraction kits and supplies, and their time-consuming characteristics

https://pubmed.ncbi.nlm.nih.gov/33376476/
The RNA extraction is an important checkpoint for the detection of SARS-CoV-2 in swab samples, but it is a major barrier to available and rapid COVID-19 testing. In this study, we validated the extraction-free RT-qPCR method by heat-treatment as an accurate option to nucleic acid purification in Alg

https://www.ohsu.edu/sites/default/files/2024-06/OCTRI_PDF_BIP_Awardees_2024.06.20.pdf
exposure from confirmed COVID-19 patients to the inside and outside surfaces of brown paper bags. PPE will be obtained from hospitalized patients and exposed health care workers during an aerosolized procedure in a COVID-19 cohort ICU. Samples will be collected from the surfaces of the PPE, the interior and exterior surfaces of the paper bag

https://www.nature.com/articles/s41580-024-00748-6
The development of high-throughput RNA structure profiling methods in the past decade has greatly facilitated our ability to map and characterize different aspects of RNA structures transcriptome