https://www.neb.com/en/tools-and-resources/video-library/brett-robb-rna-research-at-neb
My name is Brett Robb. I'm the Scientific Director for RNA research here at New England Biolabs. We work to discover, understand and develop enzymes and workflows to enable RNA research. We're currently focused on 3 main areas: New enzymes and workflows to enable messenger RNA and RNA synthesis at any scale, Enzymes and workflows that are

https://www.neb.com/en-us/tools-and-resources/video-library/brett-robb-rna-research-at-neb
We work to discover, understand and develop enzymes and workflows to enable RNA research. We're currently focused on 3 main areas: New enzymes and workflows to enable messenger RNA and RNA synthesis at any scale, Enzymes and workflows that are useful to help us understand the epitranscriptome, RNA-guided nucleases that will enable

https://www.youtube.com/watch?v=ogK7TNoxGGU
In this video, Brett Robb describes the aims and interests of the RNA Research Division at NEB. Learn more at https://www.neb.com/research/researcher-profile

https://www.linkedin.com/in/gbrettrobb
View G. Brett Robb's profile on LinkedIn, a professional community of 1 billion members. ... Passionate about scientific discovery and empowering diverse communities, I led NEB's RNA research

https://scholar.google.com/citations?user=4WWfLdoAAAAJ
2005. RNA helicase A interacts with RISC in human cells and functions in RISC loading. GB Robb, TM Rana. Molecular cell 26 (4), 523-537. , 2007. 336. 2007. The cell-specific expression of endothelial nitric-oxide synthase: a role for DNA methylation. Y Chan, JE Fish, C D'Abreo, S Lin, GB Robb, AM Teichert,

https://academic.oup.com/nar/article/51/8/3903/7103211
RNA Research Division, New England Biolabs, Inc., Beverly, MA. 01915, USA. ... Gregory Brett Robb, DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage, Nucleic Acids Research, ... rSAP (#M0371S), T4 DNA ligase (#M0202S), NEB

https://www.neb.com/en-us/tools-and-resources/video-library/a-breakthrough-method-of-rna-sample-preparation?autoplay=1
Script. Daniela Munafo: Most methods for small RNA library constructions are based on sequential ligations of adapters that leads to adapter dimer formation. Daniela Munafo: Adapter dimers can be a real problem because it strongly contaminates the library, it reduces the library yield, and increases background. Brett Robb: So there are a number of strategies to get around the adapter dimer

https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpet.36
G. Brett Robb [email protected] New England Biolabs, Ipswich, Massachusetts ... CRISPR-Cas nucleases are RNA-programmed DNA-cutting enzymes that facilitate the introduction of intentional sequence changes into the genomes of experimental cells and organisms. This overview provides a background for genome editing and CRISPR-Cas nucleases, as

https://www.nature.com/articles/s42003-022-03275-2.pdf
The Cme protein sequence is 1288 amino acids in length and shares 39.9, 31.5, and 45.2% amino-acid identity to the well-studied Lba, Asp, and FnoCas12a orthologs, respectively. Yme is 1362 amino

https://www.researchgate.net/publication/337207651_Genome_Editing_with_CRISPR-Cas_An_Overview
Genome Editing with CRISPR-Cas: An. Overview. G. Brett Robb 1,2. 1 New England Biolabs, Ipswich, Massachusetts. 2 Corresponding author: robb@neb.com. The understanding and application of clustered

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297845/
For the investigation of RNase H cleavage specificity, 0.5 μM of synthetic RNA oligonucleotides containing a 5′ FAM label were combined with 2.5 μM of the corresponding TOs in a 10 μL reaction containing 1× RNase H reaction buffer (50 mM Tris-HCl, pH 8.3, 75 mM KCl, 3 mM MgCl 2, 10 mM DTT).

https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpet.36
CRISPR-Cas nucleases are RNA-programmed DNA-cutting enzymes that facilitate the introduction of intentional sequence changes into the genomes of experimental cells and organisms. This overview provides a background for genome editing and CRISPR-Cas nucleases, as well as an overview of the technology used to assess the outcomes of genome editing

https://pubmed.ncbi.nlm.nih.gov/35680168/
The extent of 5' cap incorporation is one of the critical quality attributes in mRNA manufacturing. RNA cap analysis involves multiple steps: generation of predefined short fragments from the 5' end of the kilobase-long synthetic mRNA molecules using RNase H, a ribozyme or a DNAzyme, enrichment of the 5' cleavage products, and LC-MS intact mass

https://pubmed.ncbi.nlm.nih.gov/35388146/
CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA containing target sequences adjacent to protospacer adjacent motifs (PAM). ... Audrey Noireterre 1 2 , Peter R Weigele 1 , Zhiyi Sun 1 , G Brett Robb 3 Affiliations 1 New England ... 01938, USA. robb@neb.com. PMID: 35388146 PMCID: PMC8986864 DOI : 10.1038

https://www.neb.com/en/nebinspired-blog/adding-capabilities-to-crispr-cas-based-biotechnology
The Robb Lab at NEB collaborates with both academic and industry researchers in their mission of discovery, characterization, and application development efforts with CRISPR-Cas systems. It's always galvanizing to look to the origin of CRISPR tools and consider how this scientific field has expanded into multiple substrate types and applications over time.

https://rnajournal.cshlp.org/content/28/8/1144
The extent of 5′ cap incorporation is one of the critical quality attributes in mRNA manufacturing. RNA cap analysis involves multiple steps: generation of predefined short fragments from the 5′ end of the kilobase-long synthetic mRNA molecules using RNase H, a ribozyme or a DNAzyme, enrichment of the 5′ cleavage products, and LC-MS

https://www.nature.com/articles/s41467-020-19344-1
Fig. 1: Cas9 diversity and characterization approach. a Phylogenetic representation of the diversity provided by Cas9 orthologs. Type II-A, B, and C systems are color-coded, red, blue, and green

https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpet.36
RNA, shown in blue. Type II (Cas9) and Type V (Cas12a) RNA-guided Cas nucleases contain only one protein. Cas9 in nature uses a dual guide RNA made up of a crRNA and a tracrRNA. These RNAs can be fused into a single guide RNA for experimental purposes. Cas12a naturally uses a short single guide RNA. Sanozky-Dawes, & Barrangou, 2019; also see

https://www.nature.com/articles/s41586-024-07570-2
Although many recent studies have expanded the known universe of RNA-guided enzymes 20,21,22,23,24,25,26,27,28,29,30,31,32,33, our discovery of the IS110 bridge RNA illustrates a conceptually

https://www.neb.sg/tools-and-resources/video-library/a-breakthrough-method-of-rna-sample-preparation
Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly.We also offer solutions for automation, site-directed mutagenesis, as well as your

https://www.arounddeal.com/p/brett-g-robb/j3ymmmesav
Get detailed information about G. Brett Robb's business profile, including email address, phone number, work history, and more. Products. AroundDeal Extension. AI talents & buyers finder on LinkedIn. Prospector. ... G. Brett Robb. Scientific Director, RNA & Genome Editing

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512873/
Abstract. Clustered regularly interspaced short palindromic repeat (CRISPR) machineries are prokaryotic immune systems that have been adapted as versatile gene editing and manipulation tools. We found that CRISPR-Cas nucleases Cpf1 (also known as Cas12a) and Cas9 exhibit differential guide RNA sequence requirements for cleavage of the two

https://www.neb.com/tools-and-resources/feature-articles/crispr-cas9-and-targeted-genome-editing-a-new-era-in-molecular-biology
The rapid progress in developing Cas9 into a set of tools for cell and molecular biology research has been remarkable, likely due to the simplicity, high efficiency and versatility of the system. Of the designer nuclease systems currently available for precision genome engineering, the CRISPR/Cas system is by far the most user friendly.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164561/
INTRODUCTION. CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated protein) systems defend bacteria and archaea against invading nucleic acids (1,2).In the well-studied Type II system, the key components for nucleic acid target recognition and destruction are single, multi-domain Cas endonucleases and guide RNAs, which form RNA-endonuclease protein complexes