https://biologydictionary.net/dna-sequencing/
DNA sequencing is the process of determining the exact sequence of nucleotides within a DNA molecule. This means that by sequencing a stretch of DNA, it will be possible to know the order in which the four nucleotide bases - adenine, guanine, cytosine and thymine - occur within that nucleic acid molecule. ... DNA Sequencing Overview.

https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/dna-sequencing
DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA. In Sanger sequencing, the target DNA is copied many times, making fragments of different lengths. Fluorescent "chain terminator" nucleotides mark the ends of the fragments and allow the sequence to be determined.

https://www.khanacademy.org/test-prep/mcat/biomolecules/dna/a/dna-structure-and-function
DNA structure and function. DNA is the information molecule. It stores instructions for making other large molecules, called proteins. These instructions are stored inside each of your cells, distributed among 46 long structures called chromosomes. These chromosomes are made up of thousands of shorter segments of DNA, called genes.

https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_2e_(OpenStax)/03%3A_Unit_III-_Genetics/3.04%3A_DNA_Structure_and_Function/3.4.03%3A_DNA_Structure_and_Sequencing
The phosphate, which makes DNA and RNA acidic, is connected to the 5' carbon of the sugar by the formation of an ester linkage between phosphoric acid and the 5'-OH group (an ester is an acid + an alcohol). In DNA nucleotides, the 3' carbon of the sugar deoxyribose is attached to a hydroxyl (OH) group. In RNA nucleotides, the 2' carbon of the

https://www.ncbi.nlm.nih.gov/books/NBK26821/
A DNA Molecule Consists of Two Complementary Chains of Nucleotides. A DNA molecule consists of two long polynucleotide chains composed of four types of nucleotide subunits. Each of these chains is known as a DNA chain, or a DNA strand.Hydrogen bonds between the base portions of the nucleotides hold the two chains together ().As we saw in Chapter 2 (Panel 2-6, pp. 120-121), nucleotides are

https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/transcription-and-rna-processing/a/overview-of-transcription
It involves copying a gene's DNA sequence to make an RNA molecule. Transcription is performed by enzymes called RNA polymerases, which link nucleotides to form an RNA strand (using a DNA strand as a template). Transcription has three stages: initiation, elongation, and termination. In eukaryotes, RNA molecules must be processed after

https://en.wikipedia.org/wiki/DNA_sequencing
DNA sequencing is the process of determining the nucleic acid sequence - the order of nucleotides in DNA.It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.

https://openstax.org/books/biology-2e/pages/14-2-dna-structure-and-sequencing
Until the 1990s, the sequencing of DNA (reading the sequence of DNA) was a relatively expensive and long process. Using radiolabeled nucleotides also compounded the problem through safety concerns. With currently available technology and automated machines, the process is cheaper, safer, and can be completed in a matter of hours.

https://www.britannica.com/science/DNA-sequencing
DNA sequencing, technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid). The nucleotide sequence is the most fundamental level of knowledge of a gene or genome. ... Also, human genes are generally adjacent to so-called CpG islands—clusters of cytosine and guanine, two of the nucleotides that make up DNA. If a gene

https://www.ncbi.nlm.nih.gov/books/NBK538241/
The remarkable structure of deoxyribonucleic acid (DNA), from the nucleotide up to the chromosome, plays a crucial role in its biological function. The ability of DNA to function as the material through which genetic information is stored and transmitted is a direct result of its elegant structure. In their seminal 1953 paper, Watson and Crick unveiled two aspects of DNA structure: pairing the

https://www.nature.com/scitable/topicpage/the-order-of-nucleotides-in-a-gene-6525806/
DNA is a double-stranded, helical molecule composed of nucleotides, each of which contains a phosphate group, a sugar molecule, and a nitrogenous base. Because there are four naturally occurring

https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/dna-and-rna-structure/a/nucleic-acids
DNA sequences are usually written in the 5' to 3' direction, meaning that the nucleotide at the 5' end comes first and the nucleotide at the 3' end comes last. As new nucleotides are added to a strand of DNA or RNA, the strand grows at its 3' end, with the 5′ phosphate of an incoming nucleotide attaching to the hydroxyl group at the 3

https://www.genome.gov/genetics-glossary/DNA-Sequencing
Definition. 00:00. …. DNA sequencing refers to the general laboratory technique for determining the exact sequence of nucleotides, or bases, in a DNA molecule. The sequence of the bases (often referred to by the first letters of their chemical names: A, T, C, and G) encodes the biological information that cells use to develop and operate.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020069/
Founding Methodology. The founding methods in DNA sequencing were the Sanger dideoxy synthesis (Sanger & Coulson, 1975; Sanger, Nicklen, & Coulson, 1977) (UNIT 7.4) and Maxam-Gilbert chemical cleavage (Maxam & Gilbert, 1980) (UNIT 7.5) methods.The Maxam-Gilbert method is based on chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides.

https://biologydictionary.net/dna-structure/
DNA molecules are polymers and are made up of many smaller molecules, called nucleotides. Each nucleotide contains a phosphate group, a sugar molecule, and a nitrogenous base. DNA molecules consist of two DNA strands, which are twisted around one another to form a spiral shape known as the double helix. The double helix structure of DNA was

https://www.whatisbiotechnology.org/index.php/science/summary/sequencing/dna-sequencing-determines-the-order-of-dna-building-blocks
Nucleotides are not the only determinants of phenotypes, but are essential to their formation. Each individual and organism has a specific nucleotide base sequence. The two scientists in the photograph are reading the genetic code for a DNA sample on a highlighted light board. Such analysis is usually done by a computer.

https://www.britannica.com/science/DNA
The configuration of the DNA molecule is highly stable, allowing it to act as a template for the replication of new DNA molecules, as well as for the production (transcription) of the related RNA (ribonucleic acid) molecule.A segment of DNA that codes for the cell's synthesis of a specific protein is called a gene.. DNA replicates by separating into two single strands, each of which serves

https://www.visiblebody.com/learn/biology/dna-chromosomes/overview
These are the genes, specific regions along a strand of the DNA molecule that have particular sequences of nucleotides. These sequences are "read" by an enzyme called RNA polymerase. RNA polymerase uses a sequence of nucleotides on one strand of a DNA molecule to assemble free nucleotides into a strand of RNA. This process is called

https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/dna-and-rna-structure/v/introduction-to-nucleic-acids-and-nucleotides
Introduction to nucleic acids and nucleotides. Nucleic acids, crucial macromolecules for life, were first discovered in cell nuclei and exhibit acidic properties. DNA and RNA, composed of nucleotide building blocks, store hereditary information. These polymers have a backbone of alternating ribose and phosphate groups, with nitrogenous bases

https://byjus.com/biology/dna-structure/
The DNA is called a polynucleotide because the DNA molecule is composed of nucleotides - deoxyadenylate (A) deoxyguanylate (G) deoxycytidylate (C) and deoxythymidylate (T), which are combined to create long chains called a polynucleotide. As per the DNA structure, the DNA consists of two chains of polynucleotides. Also Read: Genetic Material

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/dna-sequence
DNA sequencing is used to determine the exact sequence of nucleotides (A, G, C, T) in a strand of DNA. The Sanger dideoxy chain-termination method can determine the sequence of nucleotides with high fidelity for a stretch of approximately 200-500 base pairs in any purified DNA sample.

https://molecular-biology.coe.hawaii.edu/lessons/content-overview-of-dna-rna-and-proteins/
Deoxyribonucleic Acid (DNA) The structural unit of DNA is the nucleotide and DNA itself is a polymer of nucleotides. A nucleotide has three basis parts: 1. One of four possible nitrogenous bases DNA: purines - adenine and guanine pyrimidine - thymine and cytosine 2. Pentose sugar called deoxyribose 3.

https://www.sciencedirect.com/topics/chemistry/dna-sequencing
DNA sequencing is the most direct method to analyse PCR products that allows for the identification of the sequence of nucleotides (A, T, C, G) of an amplicon and comparison to a reference sequence. A vast amount of genetic information is available in public databases, including a large number of sequences across a wide range of taxonomic groups.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822018/
The structure of DNA. ( A) A nucleotide (guanosine triphosphate). The nitrogenous base (guanine in this example) is linked to the 1′ carbon of the deoxyribose and the phosphate groups are linked to the 5′ carbon. A nucleoside is a base linked to a sugar. A nucleotide is a nucleoside with one or more phosphate groups.

https://www.ncbi.nlm.nih.gov/books/NBK21136/
7.1. Locating the Genes in a Genome Sequence. Once a DNA sequence has been obtained, whether it is the sequence of a single cloned fragment or of an entire chromosome, then various methods can be employed to locate the genes that are present. These methods can be divided into those that involve simply inspecting the sequence, by eye or more frequently by computer, to look for the special

https://www.sciencedirect.com/topics/immunology-and-microbiology/dna-sequence
The sequence of reactions is automatically repeated at defined intervals to yield an exponential increase in the amount of DNA. Twenty cycles of PCR amplify DNA by about a factor of 10 6 and 30 cycles by about 10 9. PCR amplification of DNA is one of the most widely used techniques in medical research and diagnostics.