ImmersiveAmbientModecolor: #b99b84 (color 1)
Video Format : 22 (720p) openh264 ( https://github.com/cisco/openh264) mp4a.40.2 | 44100Hz
Audio Format: Opus - Normalized audio
PokeTubeEncryptID: 24d7aca969927b9930e39a6145413fddce1a05ea25cfc4390588b7eccbf3bd4c7fd627af774d88131a3373aeeaa50023
Proxy : eu-proxy.poketube.fun - refresh the page to change the proxy location
Date : 1715834092860 - unknown on Apple WebKit
Mystery text : N2tKMm83UDhEMDAgaSAgbG92ICB1IGV1LXByb3h5LnBva2V0dWJlLmZ1bg==
143 : true
111,924 Views • Nov 12, 2018 • Click to toggle off description
Today we take a deep dive into the world of PCR and explore how it works, how you design the reactions, and how they're run in the lab. In a future video we may also look at how to build the thermocycler that makes this process possible, but there are lots of great tutorials on building them already online.
We also take a step forward in the spider silk project and successfully isolate 1 of the 2 genes we'll need for the project. We're still trouble shooting the other so that will be the topic of a future video. The fragment we isolated is directly usable in our construction pathway and will be inserted to build a new new plasmid we're calling pKlac2g418. This means we're only a few more steps away from finishing our plasmid and making our first spider silks!
Resources:
Spider beer overview - • Spider Beer - Making Yeast Produce Sp...
Gel electrophoresis - www.khanacademy.org/test-prep/mcat/chemical-proces…
Crash course chemistry - • The Nucleus: Crash Course Chemistry #1
Crash course biology - • Carbon... SO SIMPLE: Crash Course Bio...
The manga guide to molecular biology - www.amazon.ca/Manga-Guide-Molecular-Biology/dp/159…
Genome compiler - designer.genomecompiler.com/app
Primer3 - primer3.ut.ee/
Snap gene - www.snapgene.com/
____________________________________________________________________
Support the show and future projects:
Patreon: www.patreon.com/thethoughtemporium
Nebula: go.nebula.tv/thethoughtemporium
Ko-Fi: ko-fi.com/thoughtemporium
Become a member: youtube.com/channel/UCV5vCi3jPJdURZwAOO_FNfQ/join
Store: thethoughtemporium.ca/
______________________________________________________
Our Social Media Pages:
Tiktok: www.tiktok.com/@thethoughtemporium
Instagram: www.instagram.com/thethoughtemporium/
Facebook: www.facebook.com/thethoughtemporium/
Twitter: www.twitter.com/emporiumthought
Website: thethoughtemporium.com/
_____________________________________________________
Views : 111,924
Genre: Science & Technology
Date of upload: Nov 12, 2018 ^^
Rating : 4.979 (23/4,271 LTDR)
RYD date created : 2022-04-07T17:24:40.455293Z
See in json
YouTube Comments - 180 Comments
Top Comments of this video!! :3
Dude, you are so creative in so many different fields of science. Freaking renaissance man
63 |
Hello Justin,
I just wanted to tell you that I really love your channel, and that you've inspired me to switch from computer science to a biology major.
My entire life I KNEW I wanted to be a software engineer and I even completed a AA in IT.
But your videos (and a few others) made me realize that I have a far greater passion for plants and molecular biology.
I've enrolled at the University of Calgary majoring in plant biology, and I hope to eventually specialize in genetics.
Thank you so much for sharing your hobby and passion with the world.
80 |
My simple engineer brain finally understood PCR, thank you for this explanation.
101 |
I love that this is meant to be easily understandable. I have looked for videos on the basics of Biotech for a while, and it seems that you have taken on that mantle for all of us. Thank you.
Edit: Have you considered a video on preventing Contamination? Thanks.
88 |
Excellent video! Minor history-of-science quibble: Taq was isolated from a bug (Thermus aquaticus) in Yellowstone hot springs, while Pfu polymerase was later isolated from a deep-sea hydrothermal vent bug (Pyrococcus furiosus).
29 |
So, "polymerase chain reaction machine" sounds cool and all, but who can we talk to about getting it renamed to "gene forge"?
150 |
This world needs more people like you... love what you are doing.
2 |
Nice graphics!
Also, great vidjayo, I really liked the quick pace of it
2 |
I actually understood most of that.
43 |
I love this video because it shows practical uses for what a majority of my BIO 101 class taught me keep making your videos they are so cool.
1 |
Exelent video series. keep up the good work.
1 |
One of the best explanation in the web... Thanks
|
I've been looking for the answer on how each strand is the same length, even though extension doesn't have a stopping point beyond just cooling it down. This video gave me the answer. Thank you.
2 |
I had been waiting for this. Thanks
|
As somebody with a really messed up biology education (6 teachers in 5 years of which 2 were first-time and 1 not technically a teacher, also only until 10th grade) I really want to thank you for how understandable this video is. I was able to watch it and understand everything in one go! To be fair, I am a nerd but still: Very nice video!
12 |
You deserve far more exposure than you already get, people can really get a better understanding of Biotech from your videos, keep it up man!
2 |
I know this is late but I absolutely love how informative your videos are. I started watching this thinking I would be completely lost and lose interest. But i am glad I watched it bc I was very wrong, I always learn a lot from your channel. Thank you!
I want to get to know more of this so that I can make a lab to genetically test myself for my diagnosis of crohns possibly cure it. I also want to be able to test friends and family for diseases.
|
hey man, been waiting impatiently for your video.
7 |
Amazing how you learned everything by yourself nice job
|
@iamjimgroth
5 years ago
Oh FFS! I do not need another expensive hobby! But how can one not fall in love with this stuff?
111 |