Ion Exchange Chromatography

1 videos • 6 views • by Pharma Lecture Recording Ion exchange chromatography is a separation technique used in analytical chemistry and biochemistry to separate and purify charged molecules based on their interactions with charged stationary phases. It operates on the principle of reversible ion exchange between a solid stationary phase and a liquid/mobile phase. The stationary phase consists of a resin or gel material containing charged functional groups that attract and retain ions of opposite charge. By manipulating the mobile phase conditions, such as pH or ionic strength, ions of interest can be selectively retained or eluted from the stationary phase, enabling their separation. Ion exchange chromatography offers several advantages, including high selectivity, the ability to analyze a wide range of ionic species, versatility in handling complex samples, sensitivity for detecting trace levels of ions, and compatibility with various detection techniques. The most commonly used detector in ion chromatography is the conductivity detector, although UV-Vis spectrophotometers and mass spectrometers can also be utilized. There are different types of ion exchange chromatography, including cation exchange chromatography, anion exchange chromatography, and ion exclusion chromatography. It is important in various fields, such as water treatment, pharmaceuticals, environmental analysis, and biotechnology, where selective ion separation, purification, and analysis are crucial. Examples of ion exchange include water softening to remove hardness ions and the purification of drugs or biomolecules. Ion exchange chromatography plays a significant role in scientific research, industrial processes, and quality control, facilitating the analysis and purification of ions in diverse applications.